Resumen
Las Colecciones Biológicas son sumamente importantes para comprender los cambios que ocurren en los sistemas naturales. El Instituto de Biodiversidad Neotropical (IBN) posee una colección de insectos asociados a los ríos de Montaña de Yungas de más de 30 años, sustentada científicamente en estudios sobre sistemática y taxonomía de varios órdenes de insectos. Específicamente los tricópteros son considerados buenos indicadores de calidad de agua. El objetivo de este trabajo es darle visibilidad a la colección del IBN y accesibilidad al conjunto de datos completos de tricópteros a través de portales de libre acceso. Como resultado, la colección está representada por arriba del 50% de las especies descritas para el área de estudio. La gran proporción de los registros (38%) se colectaron entre los 500 y 1.000 msnm y provienen de los años 1999 a 2006. Los registros cubren 14 de 30 áreas protegidas, siendo el Parque Nacional Calilegua el área con mayor número de especies (19 spp.). Tres grandes áreas con escasos registros son identificadas para concentrar futuros esfuerzos de muestreos.
Métodos
Sampling description.— During the day, adult specimens were collected from ex-posed rocks and vegetation in the river using aerial nets, while nocturnal adultswere captured using a mercury vapor light trap with an electric generator from lateafternoon to early night. To enable molecular studies, mainly for immature associa-tion and phylogeny, all specimens were collected and preserved in 96% ethyl alcohol.Posterior abdominal segments of male adults were dissected and cleared in 10%NaOH at around 25 °C for a duration of 8 to 12 hours. Subsequently, each abdomenwas neutralized with lactic acid, the soft parts were removed, and the cuticle waswashed with water. The diaphanized abdomens were then preserved in 96% alcoholalong with the remaining parts of each individual. Glycerin was used as the mount -ing media to secure the different views of the genital segments and to recover theabdomen (Rueda Martín et al., 2021). The genital segments were examined underan Olympus BX 51 optical microscope equipped with a camera lucida (40X).The immature specimens were collected from rivers in northwestern Argentina,because of their wide diversity of ecological requirements, different methods wereused, such as Surber nets, D nets, and forceps with visual inspection of substrates.Larvae and pupae were fixed and preserved in 75-96 % ethyl alcohol. All associa-tions were made using the metamorphotype method (Vorhies, 1909; Milne, 1938).All collected material has been identified, labeled and georeferenced. Identificationand data are reliable, especially because all material was compared to type materi-al from National Museum of Natural History (NMNH), Smithsonian Institution,Washington, DC, USA.Data collection.— The dataset discussed here is part of the Entomological Col-lection database of the Instituto de Biodiversidad Neotropical (IBN) located in theprovince of Tucumán, Argentina. Detailed information regarding the programs usedfor analysis and dataset structure can be found in Albanesi et al. (2020).The data were analyzed in a GIS (QGIS Development Team, 2020). The geo -referenced points were overlapped on the protected areas of national and provincialjurisdiction (Administration of National Parks, Federal System of Protected Areas)and the reference maps were constructed from QGIS. In addition, digital elevationmodel GDEM-Aster V2 (global digital elevation model, version 2, Satellite Aster)was used to delineate altitude ranges that are related to the different altitude floorsof the Yungas. (1) (PDF) Trichoptera (Insecta) de la colección del Instituto de Biodiversidad Neotropical (IBN-CONICET-UNT), Tucumán, Argentina. Available from: https://www.researchgate.net/publication/372088632_Trichoptera_Insecta_de_la_coleccion_del_Instituto_de_Biodiversidad_Neotropical_IBN-CONICET-UNT_Tucuman_Argentina#fullTextFileContent [accessed Mar 14 2024].