Set de imagenes de Thecaphora frezzi obtenidas por Microscopia confocal laser

Abstract

Confocal images were acquired using a Stellaris 8 White Light Laser inverted confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with HC PL APO 10× dry (NA 0.4) and 63× oil immersion (NA 1.4) objectives. Excitation/emission wavelengths were set as follows: blue, 405/413–489 nm; green, 503/508–633 nm; red, 627/635–750 nm. The LAS Navigator software (Leica Microsystems, Germany) was used in conjunction with the 63× objective to view and merge tiles into a single composite image. Images and three-dimensional (3D) reconstructions were generated using Leica LAS X Stellaris Compass software (Leica Microsystems, Germany). Serial optical sections were taken in xyz mode to produce 3D views of the specimens, extending from the surface to the required depths.