Cinética del ATP extracelular en células Caco-2 estimuladas hipotónicamente

Abstract

The time course of [eATP] from Caco-2 cells after a hypotonic shock was quantified by luminometry performed at room temperature. (A-C) Cells were maintained in isotonic medium and, at 10 min, were exposed to isotonic medium or to hypotonic media of 180 mOsm (A), 150 mOsm (B) and 100 mOsm (C). Results are expressed as means of [eATP] from 4, 3 and 2 independent experiments run in triplicate for the 180, 150 a 100 mOsm experiments, respectively. (D) Increases in [eATP] from data in A-C were evaluated as ΔATP, i.e., the difference between [eATP] at 30 minutes post-stimulus and basal [eATP]. Cells were exposed to 300 mOsm, 180 mOsm, 150 mOsm and 100 mOsm.