Datos de investigación
Temporal analysis of pressure loss and greenhouse gas sample preservation in plastic and glass vials
Publicador:
Consejo Nacional de Investigaciones Científicas y Técnicas
Fecha de depósito:
13/04/2026
Fecha de recolectado:
1/9/2024-1/9/2025
Clasificación temática:
Resumen
We analyzed the temporal variation of methane (CH4), carbon dioxide (CO2), and nitrous oxide (N2O) concentrations in air samples of known concentration, stored for 90 days in four types of vials differing in body material, septum type, and closure system, as well as the preservation of the generated overpressure as a strategy to ensure sample integrity during storage. The selected vials included two glass and two plastic types. The glass vials were Labco Exetainer vials with polytetrafluoroethylene (PTFE)/silicone and chlorobutyl septa (G1), and vaccine-type vials with butyl stoppers and crimped aluminum seals (G2). The plastic vials were Vacutainer/EDTA-type polyethylene terephthalate (PET) vials for clinical use. Two closure configurations with rubber septa were evaluated: P1, comprising a conventional septum-only closure, and P2, which incorporated a thermoplastic elastomer (TPE) surface cover corresponding to the BD Hemogard system.
Métodos
Two complementary experiments were conducted simultaneously to asses the stability of vials used for storing greenhouse gases (GHG) samples. One of them consisted of tracking the pressure loss inside the vial over time as a conservation strategy. The other was the measurement of GHG concentration inside the vial at various intervals during a 90-day period. Two certified standard mixtures, one comprising CH4-CO2 and the other N2O, provided by the company Air Liquide were used. For each standard mixture, vial type and storage duration, three independent replicates were prepared, resulting in a total of 15 vials for each type and standard mixture. Measurements were taken at 5, 15, 30, 60, and 90 days after filling. Initially, all vials were weighted open to determine their mass at atmospheric pressure. Subsequently, they were sealed and evacuated with a vacuum system to a pressure close to 0.9 mb. Each vial was then filled with a standard GHG mixture at overpressure (exceeeding the vial’s capacity) to prevent the entering of ambient air during storage at ambient temperature (21°C). All vials were stored at room temperature and protected from direct sunlight throughout the storage period. The vials assigned to each time point were tracked cumulatively. On each measurement date, all vials were weighed, including those not yet sampled for chromatographic analysis, to record the temporal evolution of individual mass without disturbing the gas content. Immediately after weighing, on the designated analysis date, a sample was extracted from the vials assigned to that time point.
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Datos de Investigación(CIFICEN)
Datos de Investigación de CENTRO DE INV. EN FISICA E INGENIERIA DEL CENTRO DE LA PCIA. DE BS. AS.
Datos de Investigación de CENTRO DE INV. EN FISICA E INGENIERIA DEL CENTRO DE LA PCIA. DE BS. AS.
Citación
Priano, Maria Eugenia; Fusé, Victoria Susana; Juliarena, María Paula; (2026): Temporal analysis of pressure loss and greenhouse gas sample preservation in plastic and glass vials. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/284501
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