Chaotic fluctuations in mitochondrial function under oxidative stress. Superoxide dismutatase (SOD2) concentrations of 0.013, 0.016,0.0164, 0.0216733 mM

Name: Chaotic fluctuations in mitochondrial function under oxidative stress. Superoxide dismutatase (SOD2) concentrations of 0.013, 0.016,0.0164, 0.0216733 mM
License: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Keywords: CHAOS

Datos de investigación

Chaotic fluctuations in mitochondrial function under oxidative stress. Superoxide dismutatase (SOD2) concentrations of 0.013, 0.016,0.0164, 0.0216733 mM

Autores: Kembro, Jackelyn Melissa Icon

; Aon, Miguel Antonio; Cortassa, Sonia del Carmen Icon

; Lloyd, David; Sollott, Steven

Publicador: Consejo Nacional de Investigaciones Científicas y Técnicas

Fecha de depósito: 18/04/2022

Fecha de creación: 01/01/2017-31/12/2017

Clasificación temática:

Biología

Resumen

These chaotic time series of mitochondrial dynamics under conditions of oxidative stress are analyzed in detail in Kembro et al. 2018. Mitochondrial chaotic dynamics: Redox-energetic behavior at the edge of stability. Mitochondrial model used was described in the project overview and in detail (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257).

Información de Series

Numerical integration of the ME-R model equations was performed with MatCont 2.4 in MATLAB 7.1, until steady-state solutions were obtained (i.e., when the magnitude of each time derivative was -10). Time series with duration of at least 6e6 ms were constructed by numerical integration of model equations. To allow transient states to vanish, the system was computed during a time lapse of 2 e8 ms.All studies were performed using the parameter setting optimized in our previous work (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257), with ADPm = 0.01mM, i.e. consistent with energized mitochondria under state 4 respiration. The two .txt file represents the time reference and the time series of variables output of the model, in order from left to right column: 1) Mitochondrial Ca+;2) Mitochondrial ADP; 3) Membrane potential; 4)Mitocondrial NADH; 5) Mitochondrial H+; 6) Mitochondrial Phosfate, Pi; 7) Isocitrate; 8) a-ketoglutarate; 9) Succinyl CoA; 10) Succinate; 11) Fumarate; 12) Malate; 13) Oxaloacetate; 14) NADPH; 15) Mitochondrial superoxide; 16) Extramitochondrial superoxide; 17) Mitochondrial hydrogen peroxide; 18) Extramitochondrial hydrogen peroxide; 19) Mitochondrial GSH; 20) Extramitochondrial GSH; 21)Mitochondrial GSSG; 22) Mitochondrial TrxSH2; 23) ExtramitochondrialTrxSH; 24)Mitochondrial PSSGm; 25) Extramitochondrial PSSG.

Información Técnica

Model-simulated time series were calculated with 0.013, 0.016, 0.0164, or 0.0216733 mM of SOD2, Shunt=0.04, SOD1 9.7 10-5 mM. External superoxide perturbation: amplitude=1 10-7 mM, period =30 s.

Palabras clave: CHAOS, SERIE TIEMPO, MITOCONDRIA, ESTRES OXIDATIVO

Previsualización destacada

Identificador del recurso

URI: http://hdl.handle.net/11336/155280

Colecciones

Datos de Investigación(IIBYT)
Datos de Investigación de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS

Citación

Kembro, Jackelyn Melissa; Aon, Miguel Antonio; Cortassa, Sonia del Carmen; Lloyd, David; Sollott, Steven; (2022): Chaotic fluctuations in mitochondrial function under oxidative stress. Superoxide dismutatase (SOD2) concentrations of 0.013, 0.016,0.0164, 0.0216733 mM. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/155280

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