Gastrointestinal nematodes ITS-2 rDNA and β-tubulin deep amplicon sequencing from 6 cattle farms located in Buenos Aires province, Argentina

Name: Gastrointestinal nematodes ITS-2 rDNA and β-tubulin deep amplicon sequencing from 6 cattle farms located in Buenos Aires province, Argentina
License: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Keywords: NEMABIOME

Datos de investigación

Gastrointestinal nematodes ITS-2 rDNA and β-tubulin deep amplicon sequencing from 6 cattle farms located in Buenos Aires province, Argentina

Autores: Maté, María Laura Icon

; Liron, Juan Pedro Icon

; Cantón, Candela Icon

Publicador: Consejo Nacional de Investigaciones Científicas y Técnicas

Fecha de depósito: 03/04/2025

Fecha de creación: 01/04/2025

Clasificación temática:

Otras Ciencias Veterinarias

Resumen

This study establishes the first molecular identification of gastrointestinal nematodes (GIN) species parasitizing cattle across six commercial farms located in Buenos Aires province, Argentina, using ITS-2 gene deep amplicon sequencing. We further applied the fecal egg count reduction test (FECRT) and sequencing of the β-tubulin isotype-1 gene to assess BZD resistance under different anthelmintic treatment regimens.

Información Técnica

British breed cattle and their crossbreeds, representative of the beef production systems in Buenos Aires province, Argentina, were used for fecal sampling over a period of approximately two years across six commercial farms operating under a grazing-based finishing system. The efficacy of BZDs and IVM in each commercial farm included in the project was previous to the feces sampling for ITS-2 and b-tubulin analyses.In order to assess the molecular identification of GIN and determine the BZD resistance status of GIN parasitizing livestock, fecal samples from calves were collected between April 2020 and October 2021. The relative abundance of GIN species in each sample pool was determined through ITS-2 nemabiome metabarcoding following the protocol described by Avramenko et al. (2015) (https://www.nemabiome.ca/sequencing.html). The frequency of benzimidazole resistance alleles in parasitic nematodes communities was determined through deep amplicon sequencing of the isotype-1 b-tubulin fragment, as previously described and detailed in Avramenko et al. (2019). The prepared pooled library was run on an Illumina Desktop Sequencer using a 500-cycle paired-end reagent kit (MiSeq Reagent Kit, v2, MS-103–2003) at a final concentration of 15 pM, with the addition of 25% PhiX control v3 (Illumina, FC-110-3001). The MiSeq was set to generate only FASTQ files.

Palabras clave: NEMABIOME, ITS2 AND B-TUBULIN, CATTLE, PARASITES

Alcance geográfico

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Identificador del recurso

URI: http://hdl.handle.net/11336/257859

Colecciones

Datos de Investigación(CIVETAN)
Datos de Investigación de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL

Citación

Maté, María Laura; Liron, Juan Pedro; Cantón, Candela; (2025): Gastrointestinal nematodes ITS-2 rDNA and β-tubulin deep amplicon sequencing from 6 cattle farms located in Buenos Aires province, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/257859

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